EC Number   |
|---|
    1.12.1.3 | - |
| 1.12.1.3 | an active, purified 12-His tagged recombinant SHI (rSHI) is obtained by one-step affinity adsorption on nickel-charged resin |
| 1.12.1.3 | C-terminal domain of subunit HndA and N-terminal domain of subunit HndD recombinantly expressed in Escherichia coli |
| 1.12.1.3 | HndA subunit |
| 1.12.1.3 | one-step purification of the recombinant enzyme including a polyhistidine affinity tag results in a 50fold increase in yield compared to the four-step purification procedure for the native enzyme |
| 1.12.1.3 | single affinity chromatography, 4°C, aerobic conditions |
| 1.12.1.3 | strategies for reliable and improved large-scale production of Pyrococcus furiosus with integrated purification of hydrogenase I |
| 1.12.1.3 | truncated version of HndA subunit |
