EC Number |
---|
1.1.1.B4 | - |
1.1.1.B4 | native enzyme 226fold to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration |
1.1.1.B4 | native enzyme by a procedure including RedA-agarose affinity chromatography to electrophoretical homogeneity |
1.1.1.B4 | Ni Sepharose column chromatography |
1.1.1.B4 | recombinant C-terminally His-tagged mutant I86A from Escherichia coli HB101 (DE3) by nickel affinity chromatography |
1.1.1.B4 | recombinant enzyme 11fold to homogeneity from Escherichia coli strain BL21(DE3) by heat denaturation of host proteins, anion exchange chromatography and gel filtration |