EC Number   |
|---|
    1.1.1.9 | - |
| 1.1.1.9 | ammonium sulfate fractionation, DEAE Toyopearl chromatography, phenyl Toyopearl chromatography, Mono Q chromatography, Superdex 200 gel filtration, and Reactive Red column chromatography |
| 1.1.1.9 | DEAEToyopearl 650 M column chromatography, butyl-Toyopearl 650 M column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration |
| 1.1.1.9 | native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration |
| 1.1.1.9 | native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography |
| 1.1.1.9 | native enzyme by dialysis, anion exchange chromatography, ammoniuim sulfate fractionation, hydrophobic interaction chromatography, again dialysis and another different step off anion exchange chromatography, dialysis, and gel filtration, followed by repeating the last anion exchange chromatographic step. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography |
| 1.1.1.9 | native enzyme from Rhizomucor pusillus strain NBRC 4578 by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration, and a another different step of anion exchange chromatography, ultrafiltration, and gel filtration. Next purification steps are Reactive Red 120 affinity chromatography, dialysis, and ultrafiltration. Recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 1.1.1.9 | Ni-NTA column chromatography and Sephacryl S-300 gel filtration |
| 1.1.1.9 | optimized extraction by cetyl trimethyl ammonium bromide reversed micelles |
| 1.1.1.9 | partial |
