EC Number |
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1.1.1.38 | 38-40°c treatment, ammonium sulfate, protamine sulfate precipitation, calcium phosphate gel, ammonium sulfate |
1.1.1.38 | ammonium sulfate, DEAE-cellulose, Blue-B agarose, Orange-A agarose |
1.1.1.38 | DEAE Sepharose CL-6B column chromatography, and Q Sepharose column chromatography |
1.1.1.38 | MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, hydroxylapatite |
1.1.1.38 | MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, Sephadex G-200, hydroxylapatite |
1.1.1.38 | protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-cellulose |
1.1.1.38 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration |
1.1.1.38 | recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity |
1.1.1.38 | streptomycin sulfate ammonium sulfate, Phenyl-sepharose, Orange-A-Sepharose |
1.1.1.38 | streptomycin, ammonium sulfate, heat treatment, acid treatment, calcium phosphate gel, DEAE-sephadex, hydroxylapatite, Sepharose 6B |