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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357-
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357in one step using metal chelate chromatography, purity of the protein is more than 98%
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357Q-Sepharose column chromatography and Sephadex G-100 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, gel fitration, and ultrafiltration
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant enzyme from cytosol of COS-1 cells by ultracentrifugation
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant enzyme from Escherichia coli strain BL21(DE3)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography, cleavage of the tag by thrombin, and anion exchange chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant His-tagged AKR1C3 from Escherichia coli strain BL21
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant His-tagged and untagged enzyme from Escherchia coli strain BL21(DE3) nickel affinity chromatography, and by deoxycholic acid affinity chromatography, respectively, the latter to 94-98% purity. Deoxycholic acid is linked to the resin Sepharose 4B with the aid of the spacers as cyanuric chloride and ethanediamine. Adsorption analysis by adapting the adsorption isotherm to a specific binding model, the Scatchard analysis model, overview. Comparison of purification methods between synthetic affinity medium and Ni2+-Sepharose column
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
Results 1 - 10 of 14 > >>