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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite 286105
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, and tag cleavage by thrombin, followed by anion exchange chromatography, gel filtration, and dialysis 724098
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214recombinant protein 761008
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214using affinity chromatography on Red-Agarose and several subsequent ion exchange steps 347731
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