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Search Purification (Commentary)

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EC Number
Commentary
Reference
affinity chromatography using the Strep-Tag
immobilized metal ion affinity chromatography (Ni2+), gel filtration
ion exchange chromatography (Q Sepharose), gel filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain B834 by nickel affinity chromatography and cleavage of the tag by TEV protease, followed by anion exchange chromatography and gel filtration to about 95% purity
Results 1 - 5 of 5