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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 4.1.3.43Display the reaction diagram Show all sequences 4.1.3.43BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion 721651
Show all pathways known for 4.1.3.43Display the reaction diagram Show all sequences 4.1.3.43BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, although only BphJ has the histidine tag. After purification, the N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion 721615
Show all pathways known for 4.1.3.43Display the reaction diagram Show all sequences 4.1.3.43purified to homogeneity using Ni2+-NTA chromatography 721659
Results 1 - 3 of 3