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EC Number
Commentary
Reference
Ffh NG is purified
Ffh NG is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column; FtsY NGd20 is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column
glutathione Sepharose column chromatography
heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography
HiPrep heparin Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration; HiPrep heparin Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration
Ni-Sepharose column chromatography
proteins are purified using histidine-binding magnetic agarose beads
recombinant
recombinant wild-type and mutant cpSRP54 from Escherichia coli strain Rosetta BL21 by two steps of cation exchange chromatography
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