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EC Number
Purification (Commentary)
ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-300HR gel filtration
expression in Escherichia coli
expression of wild-type and mutant proteins in Escherichia coli
GCH1 in complexes with cellular proteins from organs liver, heart, brain, and kidney, by immunoaffinity chromatography, isolation of mitochondria from liver, heart and kidney
Mono Q HR column chromatography
Ni-NTA resin column chromatography
Ni2+-Sepharose column chromatography, and gel filtration
recombinant DELTA42-hGTP-CH-I from Escherichia coli M15
recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography
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