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EC Number
; nickel resin chromatography
; recombinant wild-type and inactive mutant E227A
anion exchange chromatography and gel filtration
His-TRAP chelating nickel affinity column chromatography
native enzyme from soft enamel-alkaline extract by anion exchange chromatography, ultrafiltration, and heparin affinity chromatography
Ni-NTA column chromatography
purified on Ni column
Q-Sepharose column chromatography and heparin Sepharose 6 column chromatography
recombinant from Escherichia coli
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