EC Number   |
Reference  |
|---|
   3.4.22.33 | - |
30273, 30274, 30296, 30299, 710318 |
| 3.4.22.33 | fractionation |
30277, 30278 |
| 3.4.22.33 | fruit bromelain A, B and C |
30277 |
| 3.4.22.33 | 2 forms: FBA and FBB |
30278 |
| 3.4.22.33 | reverse micellar extraction system with cetyltrimethylammonium bromide |
681047 |
| 3.4.22.33 | CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration |
682716 |
| 3.4.22.33 | bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction |
717487 |
| 3.4.22.33 | high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit |
717625 |
| 3.4.22.33 | using centrifugation, salt precipitation technique, dialysis, ion-exchange chromatography. Ion exchange chromatography using diethylaminoethyl cellulose (DEAE) anion exchangers maintain the structural integrity of purified bromelain and thereby the product exhibits better proteolytic activity than crude extract |
718411 |
| 3.4.22.33 | native enzyme 2.24fold |
753642 |
