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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number
Commentary
Reference
2 forms: FBA and FBB; fractionation
bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
fractionation; fruit bromelain A, B and C
high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit
reverse micellar extraction system with cetyltrimethylammonium bromide
using centrifugation, salt precipitation technique, dialysis, ion-exchange chromatography. Ion exchange chromatography using diethylaminoethyl cellulose (DEAE) anion exchangers maintain the structural integrity of purified bromelain and thereby the product exhibits better proteolytic activity than crude extract
Results 1 - 8 of 8