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Results 1 - 9 of 9
EC Number
Commentary
Reference
HiTrap SP column chromatography, and G75 Sephadex gel filtration
native mature Spe B by ammonium sulfate fractionation, dialysis, and ion exchange chromatography, recombinant His-tagged Spe B propeptide and SpeB mutant C47S from Escherichia coli
Ni2+-chelating column chromatography
recombinant His-tagged wild-type and mutant C192S enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the wild-type enzyme autoprocesses during purification to the mature 28 kDa protein
recombinant pro-sequence domain and refolded mature enzyme from Escherichia coli by affinity chromatography
recombinant wild-type and inactive mutant enzyme from Escherichia coli by affinity chromatography
the proteins are purified by Ni2+-chelating chromatography with a gradient of 20-200 mM imidazole. The proteins are concentrated by ultrafiltration using a 10 kDa cutoff membrane and then exchanged with phosphate-buffered saline
using Ni-NTA chromatography
Results 1 - 9 of 9