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Search Purification (Commentary)

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EC Number
Commentary
Reference
recombinant His-tagged WpbE from Escherichia coli strain BL21-CodonPlus(DE3)RIL by nickel affinity chromatography
recombinant His6-tagged WbpE from Escherichia coli strain Rosetta(DE3)
recombinant N-terminal His6-tagged WbpE from Escherichia coli BL21-CodonPlus(DE3)RIL by nickel affinity chromatography, the N-terminal His6-tag is removed by proteolysis with TEV protease over the course of three days while stirring in dialysis buffer, followed by gel filtration
Results 1 - 3 of 3