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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number
Commentary
Reference
centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant
gel filtration, Western Blot, heterologously expressed protein
native enzyme at pH 6.4, by anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity
native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl
partially
recombinant enzyme
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity
Results 1 - 8 of 8