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Search Purification (Commentary)

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EC Number
Commentary
Reference
affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification; affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification; labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification
affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification; affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification; labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification; solubilization of membranes and centrifugation, chromatography on DEAE-column and Mono-Q column later on, quantitative information
enzyme transfected in HEK-293T cells partially purificated by solubilization and centrifugation
histidine-tagged truncated LRAT
native enzyme partially by membrane preparation
native enzyme partially by membrane preparation from retinal pigment epithelium
native enzyme partially by microsome preparation from eyes, recombinant enzyme partially from HEK-293T cells by membrane preparation
recombinant His-tagged LRAT by affinity chromatography
recombinant His-tagged truncated wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography in presence of 1% SDS
recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity
Results 1 - 10 of 14 > >>