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Search Purification (Commentary)

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EC Number
Commentary
Reference
native enzyme
native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration
native enzyme partially from young leaves by ammonium sulfate fractionation, gel filtration, and ultrafiltration
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration
using the His-tagged affinity chromatography
Results 1 - 5 of 5