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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 1.2.1.39Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.39- 390292, 390300
Show all pathways known for 1.2.1.39Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.39by gel filtration, 15.9fold with a 0.21% yield 690567
Show all pathways known for 1.2.1.39Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.39native enzyme 126fold by cross-flow filtration, ultracentrifugation, and anion exchange chromatography, recombinant Strep-tagged enzyme 137fold from Escherichia coli strain DHalpha by affinity chromatography on a Strep-Tactin column 742786
Show all pathways known for 1.2.1.39Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.39Ni-affinity column chromatography 762624
Show all pathways known for 1.2.1.39Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.39recombinant N-terminally His-tagged enzyme, NPADH, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to over 90% purity 741787
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