by affinity chromatography
by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity
by fast protein liquid chromatography on hydroxylapatite and on AH-Sepharose with covalently bound 4-hydroxy-a cyanocinnamic acid; hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography