Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Purification (Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

<< < Results 11 - 16 of 16
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity 658001
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135recombinant truncated tLRAT and its S175R mutant 719418
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability. Detergents such as 0.2% Triton X-100, 0.7% CHAPSO,and 1.2 mM n-dodecyl-beta-D-maltoside and sodium are not effective 735707
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135solubilization of membranes and centrifugation, chromatography on DEAE-column and Mono-Q column later on, quantitative information 638617
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135solubilization of membranes and centrifugation, chromatography: mono Q column and Green 5-agarose column 638611
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.135solubilization, centrifugation and dialysis 638623
<< < Results 11 - 16 of 16