EC Number |
Reference |
---|
2.3.1.135 | recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity |
658001 |
2.3.1.135 | recombinant truncated tLRAT and its S175R mutant |
719418 |
2.3.1.135 | SDS is necessary for tLRAT extraction from cell lysates. tLRAT enzymatic activity drastically diminishes at SDS concentrations below 0.05% and remains unchanged when SDS concentration is increased from 0.05 to 1%. SDS is very important for tLRAT stability. Detergents such as 0.2% Triton X-100, 0.7% CHAPSO,and 1.2 mM n-dodecyl-beta-D-maltoside and sodium are not effective |
735707 |
2.3.1.135 | solubilization of membranes and centrifugation, chromatography on DEAE-column and Mono-Q column later on, quantitative information |
638617 |
2.3.1.135 | solubilization of membranes and centrifugation, chromatography: mono Q column and Green 5-agarose column |
638611 |
2.3.1.135 | solubilization, centrifugation and dialysis |
638623 |