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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) to homogeneity by nickel affinity chromatography and two different steps of gel filtration 761687
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration 739791
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) codon plus-RIPL by heat treatment at 90°C for 10 min, nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration 741408
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3recombinant separated catalytic domains 654640
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3separation of the two isozymes using Blue-Sepharose chromatography 246382
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3separation of the two isozymes using Matrex Gel Red A affinity chromatography 246382
Show all pathways known for 1.1.1.3Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.3threonine sensitive and threonine insensitive isozymes 246385
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