EC Number |
Posttranslational Modification |
Reference |
---|
2.3.1.43 | glycoprotein |
- |
487028, 661231, 757930 |
2.3.1.43 | glycoprotein |
13% hexoses, 6.2% hexosamines |
486985 |
2.3.1.43 | glycoprotein |
21% w/w carbohydrate, including hexoses, hexosamines and sialic acids |
486989, 486991 |
2.3.1.43 | glycoprotein |
24% carbohydrate by weight, 13 mol of mannose, 30 mol of galactose, 17 mol of glucosamine and 13 mol of sialic acid per mol of enzyme protein |
486978, 487007 |
2.3.1.43 | glycoprotein |
25% carbohydrate |
486981, 486985 |
2.3.1.43 | glycoprotein |
a recombinant C-terminal histidine tagged enzyme has similar carbohydrate moieties than the enzyme purified from plasma |
487023 |
2.3.1.43 | glycoprotein |
almost all of the carbohydrate moiety of LCAT is N-linked with part of the high-mannose and part of the complex type |
486988 |
2.3.1.43 | glycoprotein |
almost all of the N-linked glycans in human LCAT are fucosylated and sialylated. The predominant LCAT N-linked glycoforms are biantennary glycans, followed by triantennary sugars, whereas the level of tetraantennary glycans is much lower. Glycans at the Fc N-linked site exclusively contain typical asialobiantennary structures. Recombinant human lecithin-cholesterol acyltransferase Fc fusion chimeric protein is also confirmed to have mucin-type glycans attached at T407 and S409. When LCAT-Fc fusions are constructed using a G-S-G-G-G-G linker, an unexpected 1632 Da xylose-based glycosaminoglycan (GAG) tetrasaccharide core of Xyl-Gal-Gal-GlcA is attached to S418. Several minor intermediate species including Xyl, Xyl-Gal, Xyl-Gal-Gal, and a phosphorylated GAG core are also present. E416 (the C-terminus of LCAT) combined with the linker sequence is likely serving as a substrate for peptide O-xylosyltransferase |
737203 |
2.3.1.43 | glycoprotein |
analysis of glycosylation pattern, glycosylation e.g. at residues Asn20, Asn84, and Asn272, modification of the glycan side chains by use of drugs and by other enzymes, physiological function, glycosylation pattern of the recombinant enzymes differs from the wild-type, overview |
661432 |
2.3.1.43 | glycoprotein |
analysis of sugar composition, overview |
663302 |