EC Number |
Application |
Reference |
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5.3.1.27 | synthesis |
engineering of a strain of Corynebacterium glutamicum, which is able to produce the polyamide building block cadaverine as non-native product, for coutilization of methanol by expression of the gene encoding NAD+-dependent methanol dehydrogenase (Mdh) from Bacillus methanolicus, deletion of the endogenous aldehyde dehydrogenase genes Ald and FadH and expression of genes for hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase of the ribulose monophosphate pathway. Growth with methanol as sole carbon source is not observed, but in vivo activity of the ribulose monophosphate pathway |
-, 746885 |
5.3.1.27 | synthesis |
engineering of Corynebacterium glutamicum toward the utilization of methanol as an auxiliary carbon source in a sugar-based medium by heterologous expression of a methanol dehydrogenase from Bacillus methanolicus, and implementation of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerasehe of Bacillus subtilis. The recombinant strain shows an average methanol consumption rate of 1.7 mM/h in a glucose-methanol medium, and the culture grows to a higher cell density than in medium without methanol |
-, 746827 |
5.3.1.27 | synthesis |
production of D-[1-13C]fructose 6-phosphate from 13C-enriched formaldehyde and D-ribose 5-phosphate using 3-hexulose 6-phosphate synthase and 6-phospho-3-hexuloisomerase from Methylomonas aminofaciens and spinach ribose 5-phosphate isomerase |
-, 685642 |