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Results 1 - 4 of 4
EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.8analysis estimation of the content of L-hydroxyprolines using coupling systems with metabolic enzymes of the trans-4-hydroxy-L-proline pathway (hydroxyproline 2-epimerase (HypE) and cis-4-hydroxy-D-proline dehydrogenase (HypDH)) and the trans-3-hydroxy-L-proline pathway (trans-3-hydroxy-L-proline dehydratase (T3LHypD) and DELTA1-pyrroline-2-carboxylate reductase (Pyr2CR)) from microorganisms. A functional expression system of recombinant HypDH with a heterooligomeric structure is constructed in Escherichia coli cells. Enzymological characterization reveals that the beta-subunit acts as a catalytic subunit, and also that assembly with other subunit(s) improves the kinetics for cis-4-hydroxy-D-proline and thermostability. By using a spectrophotometric assay with different wavelengths, the contents of trans-4-hydroxy-L-proline and trans-3-hydroxy-L-proline are successfully estimated within the ranges of 0.004-1 mM and 0.05-1 mM, respectively, and are consistent with the contents determined by HPLC. This enzymatic method is used to measure the content of trans-4-hydroxy-L-proline in the acid-hydrolysate of collagen, and blood plasma 748266
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.8analysis spectrophotometric assay for hydroxyproline in collagenous tissue hydrolysates, with an enzymatic method using 4-hydroxyproline 2-epimerase,EC 5.1.1.8, D-amino acid oxidase, EC 1.4.3.3, and a colorimetric reagent of the mixture of Ti(IV) and 4-(2-pyridylazo)-resorcinol 2161
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.8medicine PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity -, 748663
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.8molecular biology PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity -, 748663
Results 1 - 4 of 4