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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.20analysis bacteriophage lambda protein phosphatase and recombinant RNase U2 can be used to generate RNase digestion products amenable to liquid chromatography-tandem mass spectrometry analysis, which can be used to determine the presence and location of modified ribonucleosides in RNA samples 749558
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.20analysis improvement of RNA modification mapping sequence coverage by LC-MS. The non-specific ribonuclease activity RNase U2 E49A substitution mutant provides increased sequence coverage of substrate RNA during modification mapping. Data analysis software is modified to account for non-specific digestion. This combination allows efficient and accurate RNA modification mapping 749569
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