EC Number   |
Application |
Reference |
|---|
    4.2.2.20 | biotechnology |
bond strength of two etch-and-rinse adhesives to chondroitinase ABC treated dentin is investigated. Human extracted molars are treated with chondroitinase ABC. Increased mean values of microtensile bond strength and reduced nanoleakage expression are shown for both adhesives after chondroitinase ABC treatment of the dentin surface. This study supports the hypothesis that adhesion can be enhanced by removal of chondroitin 4/6 sulfate and dermatan sulfate, probably due to a reduced amount of water content and enlarged interfibrillar spaces |
693269 |
| 4.2.2.20 | medicine |
adult rats that undergo unilateral cervical spared-root lesion exhibit profound sensory deficits for 4 weeks after injury. Delivery of a focal intraspinal injection of the chondroitin sulfate proteoglycan-degrading enzyme chondroitinase ABC (ChABC) is sufficient to restore sensory function after lesion. In vivo electrophysiological recordings confirm that behavioral recovery observed in ChABC-treated rats is consequent on reorganization of intact C7 primary afferent terminals and not regeneration of rhizotomized afferents back into the spinal cord within adjacent segments. These data confirm that intact spinal circuits have a profound influence on functional restoration after spinal cord injury |
693749 |
| 4.2.2.20 | medicine |
antisense vimentin cDNA and ChABC are administered to hemisected rat spinal cords. Using RT-PCR, Western blotting and immunohistochemistry, it is shown that the combined treatment reduces the formation of glial scar and cystic cavities through degrading chondroitin sulfate proteoglycans molecules and inhibiting intermediate filament proteins |
690770 |
| 4.2.2.20 | medicine |
cervical dorsal column injury and treatment with chondroitinase ABC treatment leads to robust sprouting of both injured and intact descending projections as well as uninjured primary afferents. Enzyme treatment of uninjured animals does not induce sprouting in any system, so both denervation and chondroitin sulfate proteoglycan degradation are required to promote sprouting within the spinal cord. No increase in connectivity of nociceptive neurons or development of mechanical allodynia or thermal hyperalgesia |
681541 |
| 4.2.2.20 | medicine |
development of thermostabilized chondroitinase ABC and a system for its sustained local delivery in vivo, obviating the need for chronically implanted catheters and pumps. Presence of with 1M trehalose significantly enhances chondroitinase ABC thermal stability and prolongs enzyme activity. Thermostabilized chondroitinase ABC remains active at 37°C in vitro for up to 4 weeks. Chondroitin sulfate proteoglycan levels remain low in vivo up to 6 weeks post-spinal cord injury when thermostabilized chondroitin sulfate proteoglycan ABC is delivered by a hydrogel-microtube scaffold system. Axonal growth and functional recovery following the sustained local release of thermostabilized chondroitinase ABC versus a single treatment of unstabilized chondroitinase ABC demonstrate significant differences in chondroitin sulfate proteoglycan digestion. Animals treated with thermostabilized chondroitinase ABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers |
706560 |
| 4.2.2.20 | medicine |
effects of degrading chondroitin sulfate glycosaminoglycan with Ch'ase ABC in the injured spinal cords of adult cats is assessed. Recovery of skilled locomotion (ladder, peg, and beam) is significantly accelerated in Ch'ase ABC-treated cats compared to controls. Ch'ase ABC-treated cats also show greater recovery of specific skilled locomotor features including intralimb movement patterns and significantly greater paw placement onto pegs. These findings show that intraspinal cleavage of CS GAGs can enhance recovery of function following spinal cord injury in large animals with sophisticated motor behaviors and axonal growth requirements similar to those encountered in humans |
692234 |
| 4.2.2.20 | medicine |
immediate and long-term effects of a single injection of chABC on chondroitin sulphate proteoglycans, chondroitin sulphate glycosaminoglycan and axon regeneration after unilateral nigrostriatal lesions in adult rats are investigated. In chABC-treated animals, the total chondroitin sulphate glycosaminoglycan remain at low level throughout the 28 day experimental period. This suggests the persistence of active chABC for at least 10 days after injection which is able to digest chondroitin sulphate proteoglycans released from cells during this time. Results suggest that a single injection of chABC can produce an environment conducive to CNS repair for over 10 days |
693724 |
| 4.2.2.20 | medicine |
incubation of immature articular cartilage in serum-supplemented medium results in expansive growth with a marked increase in tissue volume associated with diminution of tensile integritiy. Treatment with chondroitinase ABC on day 0 leads to a marked reduction of glycosaminoglycan content and enhancement of tensile integrity. Subsequent incubation with enzyme leads to maturational growth with minimal changes in tissue volume and maintenance of tensile intregrity at the enhanced levels |
677880 |
| 4.2.2.20 | medicine |
intraspinal delivery of chondroitinase ABC, following T10 hemisections in adult cats, enhances adaptive movement features during a skilled locomotor task and/or promotes plasticity of spinal and supraspinal circuitry. Chondroitinase ABC enhances crossing of a peg walkway post-spinal cord injury and significantly improves ipsilateral hindlimb trajectories and integration into a functional forelimb-hindlimb coordination pattern. Recovery of these complex movements is associated with significant increases in neurofilament immunoreactivity immediately below the spinal cord injury in the ipsilateral white and contralateral gray matter. Significantly more retrogradely labeled right axotomized red nucleus neurons are seen in chondroitinase ABC-treated compared with control-treated cats indicating that a larger number of red nucleus neurons in chondroitinase ABC-treated cats had axons below the lesion level |
715993 |
| 4.2.2.20 | medicine |
it is shown that cervical spinal cord injury (SCI) results in the significantly increased expression of the chondroitin sulfate proteoglycans (CSPGs) NG2, neurocan, and brevican in the distant denervated dorsal column nuclei (DCN). The CSPGs present a potent barrier to reinnervation by regenerating axons from microtransplanted adult sensory neurons following SCI that can be overcome by chondroitinase ABC (chABC) application and increased neurotrophin-3 (NT-3) expression within the nucleus |
692233 |
