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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.28degradation possible use in anaerobic polyethylene glycol degradation 5633
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.28synthesis production of 2-butanol, using a TEV-protease based expression system to achieve equimolar expression of the B12-dependent dehydratase subunit and use of NADH-dependent secondary alcohol dehydrogenase Sadh from Gordonia sp. able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 460.2 mg/l 2-butanol and 260.1 mg/l of butanone is found. Availability of NADH is achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions 749131
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