  4.1.2.28 | synthesis |
biosynthesis route for ethylene glycol production from D-xylose, consisting of conversion steps D-xylose to D-xylonate to 2-dehydro-3-deoxy-D-pentonate to glycoaldehyde to ethylene glycol. Implementation of the respective enzymes, D-xylose dehydrogenase, D-xylonate dehydratase, 2-dehydro-3-deoxy-D-pentonate aldolase, and glycoaldehyde reductase, in a metabolically engineered Escherichia coli, in which the D-xylose to D-xylulose reaction is prevented by disrupting the D-xylose isomerase gene, leads to a construct producing 11.7 g/l of ethylene glycol from 40.0 g/l of D-xylose |
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