EC Number   |
Application |
Reference |
|---|
   3.5.4.23 | biotechnology |
the enzyme is a selection marker for genetic transformation of the diatom Phaeodactylum tricornutum. An easy handle system of antibiotic selection with blasticidin-S and the resistance gene bsr in Phaeodactylum tricornutum is reported. Blasticidin-S works by blocking protein translation and therefore it is not expected to affect the genomic DNA of transformed cells. Especially for reverse genetics studies via genome editing, when phenotypes should be determined based on cell-linages with as less off-target genetic changes as possible, utilization of this antibiotic is a major improvement. Additionally, blasticidin-S selection can be used in combination with nourseothricin- and potentially also with Zeocin-resistant cells. This extension of the genetic toolbox can be useful for further molecular characterization or biotechnological application of the model diatom Phaeodactylum tricornutum and possibly for other species of diatoms |
757914 |
| 3.5.4.23 | molecular biology |
BSD gene will be useful as a new dominant selectable marker for eukaryotes, first sucessful transformation with a drug resistance gene originating from a eukaryote by selecting for detoxification of the drug |
209573 |
| 3.5.4.23 | molecular biology |
generation of bicistronic expression vector mediating BSD resistance, blastidicin S selection of cells |
697727 |
| 3.5.4.23 | molecular biology |
new selection marker for transformation of Arabidopsis thaliana and Nicotiana tabacum |
209574 |
