    3.5.2.10 | analysis |
construction of a biosensor by co-immobilization of creatininase, creatinase, and sarcosine oxidase onto iron oxide nanoparticles/chitosangraft-polyaniline, Fe3O4-NPs/CHIT-g-PANI, composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling. The creatinine biosensor uses enzymes/Fe3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The biosensor exhibits an optimum response within 2 s at pH 7.5 and 30°C, when polarized at 0.4 V versus Ag/AgCl. The electrocatalytic response shows a linear dependence on creatinine concentration ranging from 1 to 800 microM. The sensitivity of the biosensor is 3.9 microA per microM and cm2, with a detection limit of 1 microM. The biosensor shows only 10% loss in its initial response after 120 uses over 200 days, when stored at 4°C. The biosensor measures creatinine in the serum of apparently healthy persons |
719377 |
