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3.1.5.B1
analysis
high-throughput colorimetric assay for dNTP hydrolase activity, using Escherichia coli inorganic pyrophosphatase to convert triphosphate to 3 phosphates
751132
3.1.5.B1
analysis
high-throughput screen that directly measures SAMHD1 catalytic activity, utilizing bis(4-nitrophenyl) phosphate as the substrate and Mn2+ as the activating cation
749936
3.1.5.B1
medicine
in fibroblasts of patients with genetic mutations of deoxyguanosine kinase, SAMHD1 leads to manifestation of dNTP pool imbalance and DNA depletion. When SAMHD1 is silenced by siRNA transfection the composition of the mitochondrial dNTP pool approaches that of the controls, and DNA copy number increases
751048
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