EC Number |
Application |
Reference |
---|
3.1.16.1 | analysis |
broadly applicable, robust, and rapid method for complete sequence confirmation of highly modified oligonucleotides containing a mixture of 2'-deoxy, 2'-fluoro, 2'-O-methyl, abasic and ribonucleotides. The sense and antisense strands from synthetic short interfering RNA duplexes are digested individually using both 5'- and 3'-exonucleases and the resulting ladders are analyzed using MALDITOF mass spectrometry. Complete sequence confirmation for the antisense strands of four synthetic RNA duplexes is obtained, whereas a three-base sequence gap in the 5'-end is observed for all four sense strands. Outline of a general strategy for routine sequence confirmation of highly modified oligonucleotides |
710529 |
3.1.16.1 | analysis |
detection and assay of RNA-linked nascent DNA pieces in E.coli strains |
134158 |
3.1.16.1 | analysis |
end group and sequential analysis of polynucleotides |
134140, 134149, 134151 |
3.1.16.1 | analysis |
kinetic studies on macromolecular substrates degradation |
134134 |
3.1.16.1 | more |
Ape2 exhibits strong 3'-5' exonuclease and 3'-phosphodiesterase activities and has only a very weak AP-endonuclease activity |
682165 |
3.1.16.1 | more |
important role for TRM2 in DNA repair with a potential involvement of its nuclease function in homologous recombination based repair of DNA double-strand breaks, plays no role in the nucleotide excision repair and/or base excision repair pathways |
681898 |
3.1.16.1 | more |
Plasmodium FEN-1s have enzymatic activities similar to other species but contain extended C-termini and a more internally located proliferating cell nuclear antigen-binding site. FEN-1 homologs exhibit both endonuclease and exonuclease activities in vitro |
-, 681823 |
3.1.16.1 | more |
XRNA and XRND are required for trypanosome growth |
682836 |