EC Number   |
Application |
Reference |
|---|
    2.8.3.1 | analysis |
amperometric propionate sensor |
694994 |
| 2.8.3.1 | biotechnology |
establishing of an on-site monitoring of volatile fatty acids VFA, such as propionate, biosensing system using recombinant propionate CoA transferase and recombinant acyl-CoA oxidase, this system produces hydrogen peroxide in the presence of acetyl-CoA, oxygen, and VFA substrates, which can be quantified by colorimetric methods using peroxidase and dye reagents, e.g., 4-aminobenzoic acid plus 4-aminoantipyrine, overview |
672769 |
| 2.8.3.1 | biotechnology |
propionate-biosensor, using propionate coenzyme A transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana as enzyme electrodes |
694994 |
| 2.8.3.1 | synthesis |
a platform pathway for the production of widely used industrial chemicals 1,3-diols, e.g. 1,3-pentanediol, is engineered in Escherichia coli. The pathway is designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, bld (butyraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and the endogenous alcohol dehydrogenase(s) of Escherichia coli. The recombinant Escherichia coli strains produce 1,3-pentanediol, 4-methyl-1,3-pentanediol, and 1,2,4-butanetriol, together with 1,3-butanediol, from mixtures of glucose and propionate, isobutyrate, and glycolate, respectively, in shake flask cultures |
760715 |
| 2.8.3.1 | synthesis |
engineered mutant Pct, coexpressed with an engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1, is able to produce poly(3-hydroxybutyrate-co-lactate) copolymers having 9-64 mol% of lactate. Polylactic acid might be a good alternative to petroleum-based plastic as it possesses several desirable properties such as biodegradability, biocompatibility, compostability, and low toxicity to humans |
702792 |
| 2.8.3.1 | synthesis |
in an engineered Escherichia coli strain JLX7, the engineered enzyme mutant Pct540, coexpressed with an engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1, is able to effectively produce polylactic acid, a promising biomass-derived polymer, overview |
702793 |
