EC Number   |
Application |
Reference |
|---|
   2.7.7.B22 | analysis |
mutant K212R/P214R/G251R/A338V, i.e. Tn5-059, displays a lowered GC insertion bias. Tn5-059 reduces AT dropout and increases uniformity of genome coverage in both bacterial genomes and human genome. Tn5-059 generates a higher library diversion when compared to Nextera v2 for human exomes. When used for human exomes, Tn5-059 delivers consistent library insert size over a range of input DNA, allowing up to a tenfold variance from the 50 ng input recommendation |
760785 |
| 2.7.7.B22 | analysis |
Tn5 transposase is capable of direct tagmentation of RNA/DNA hybrids in vitro. This activity can be used to replace the traditional library construction procedure of RNA sequencing. Results of transposase-assisted RNA/DNA hybrids Co-tagmEntation are compared to traditional RNA-seq methods in terms of detected gene number, gene body coverage, gene expression measurement, library complexity, and differential expression analysis |
760982 |
| 2.7.7.B22 | biotechnology |
application of a hyperactive transposase variant to generate mutants with integrated genes for the expression of the superfolder green fluorescent protein gene or a 2-ketodecarboxylase gene in Acidithiobacillus ferrooxidans, which enables the production and secretion of isobutyric acid. An inverse PCR method identifies the insertion sites of the 2-ketodecarboxylase gene, i.e. functional exogenous metabolic genes have been chromosomally integrated |
760393 |
| 2.7.7.B22 | medicine |
an hyperactive variant with enhanced solubility and stability can be delivered with transposon DNA to genetically modify cell lines and embryonic, hematopoietic and induced pluripotent stem cells. The variant is used to generate chimeric antigen receptor T-cells, which exhibit potent anti-tumor activity in vitro and in xenograft mice. The variant spontaneously penetrates cells, enabling modification of induced pluripotent stem cells and generation of generate chimeric antigen receptor T-cells without the use of transfection reagents |
761966 |
| 2.7.7.B22 | medicine |
plasmid based delivery of the sleeping beauty transposase SB100X system reveals significantly increased integration efficiencies compared with the hyperactive sleeping beauty transposase HSB5. Up to eightfold and 100fold increased integration efficiencies are observed compared with the hyperactive SB transposase HSB5 and the inactive transposase mSB, respectively. Transposon copy numbers per cell are doubled with SB100X compared with HSB5. This hybrid vector system represents a tool for achieving stabilized transgene expression in cycling cells and for treatment of numerous genetic disorders |
761945 |
| 2.7.7.B22 | medicine |
Sleeping Beauty (SB) transposon is a nonviral gene transfer vector, already used in clinical trials. Full-length dysferlin transfer by the hyperactive sleeping beauty transposase restores dysferlin-deficient muscle, which can be used for nonviral gene delivery of full-length human dysferlin into muscle cells, along with a successful and efficient transplantation into skeletal muscle, important advances towards successful gene therapy of dysferlin-deficient muscular dystrophy |
739122 |
| 2.7.7.B22 | medicine |
strategy to genetically engineer cancer patient T cells with cell receptors (TCRs) using the clinical Sleeping Beauty transposon/transposase system. Patient-specific TCRs are assembled with murine constant chains and cloned into Sleeping Beauty transposons. Patient peripheral blood lymphocytes are coelectroporated with SB11 transposase and Sleeping Beauty transposon, and transposed T cells are enriched by sorting on murine TCRbeta expression. Transposed T cells specifically mount a polyfunctional response against cognate mutated neoantigens and tumor cell lines |
761944 |
| 2.7.7.B22 | molecular biology |
Sleeping Beauty is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends |
739149 |
| 2.7.7.B22 | molecular biology |
the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences and hence are important functional genomics tool for tagging and cloning of unknown gene sequences |
739041 |
| 2.7.7.B22 | molecular biology |
transposases are important tools in genome engineering. The first DNA transposon tool capable for gene transfer in vertebrates is Sleeping Beauty (SB), which is reconstructed from extinct Tc1/mariner transposons in fish. Sleeping Beauty, and especially its hyperactive variant is still one of the most widely used transposon tools, in human clinical trials |
739123 |
