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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.64molecular biology polyclonal rabbit anti-AtUSP antibody recognizes USP1 and USP2 694741
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.64synthesis in mammals, uridine 5'-diphosphate-glucose (UDP-Glc) and uridine 5'-diphosphate-glucuronic acid (UDP-GlcA) are common building blocks of glycans and glycoconjugates. The commercial demand for these high-energy donors is increasing. To produce valuable UDP-GlcA in a cost-effective way, UDP-sugar pyrophosphorylase from Arabidopsis thaliana is constitutively expressed in Pichia pastoris and secreted into the extracellular medium. The synthesis of 4.2 g UDP-GlcA or 5.5 g UDP-Glc per liter of culture is revealed in the culture medium, without any need for purification. An anion exchange chromatography purification method for UDP-sugars is also developed. This route opens a door to large-scale production of the cheaper UDP-GlcA 760379
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.64synthesis the enzyme is useful for the highly efficient production of UDP-galacturonic acid for studies on pectin biosynthesis 667193
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.64synthesis use of UDP-sugar pyrophosphorylase (USP) from Arabidopsis thaliana with a galactokinase from Streptococcus pneumoniae TIGR4 (SpGalK) and an inorganic diphosphatase (PPase) to effectively synthesize UDP-sugars 737817
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