EC Number   |
Application |
Reference |
|---|
   2.7.7.31 | analysis |
construction of a nonhomologous end-joining assay vector NAV, containing mKate2, Venus and ccdB genes. Cotransfection of NAV with a construct expressing the restriction enzyme I-SceI generates a double-strand break in NAV that excises mKate2 and ccdB. Repair of this double-strand break produces an intact vector that expresses Venus, a green fluorescent protein. Cells bearing the repaired NAV lack the ccdB gene which slows cell proliferation, the cultures are enriched in cells containing repaired double-strand breaks |
738253 |
| 2.7.7.31 | analysis |
DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates. Amino- and nitrophenyl-modified dNTPs are good substrates giving 3'-end stretches of different lengths depending on the nucleotide and concentration. d[7-Deaza-7-(3-nitrophenyl)]GTP is efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide, resulting in a considerable enhancement of voltammetric signals due to the nitro group reduction. Discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes is easily accomplished, and tumour suppressor p53 protein is able to recognize a specific binding site within tail-labelled DNA substrates |
723321 |
| 2.7.7.31 | analysis |
methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase TdT using a thioflavin T probe. The method is highly selective and sensitive. The fluorescence intensity is directly proportional to Dam MTase concentration in the range from 0.1 to 8.0 U/ml with a detection limit of 0.1 U/ml. As no labeling with a fluorophore quencher pair is required, the method is simple and low cost |
739061 |
| 2.7.7.31 | analysis |
reliable and accurate 3'-end miRNA-labeling method for microarray detection by terminal-deoxynucleotidyl transferase TdT. Using its ability to add polynucleotides at a RNA receptor molecule by using deoxycytidine triphosphate, miRNA is successfully labeled by adding fluorescence deoxycytidine triphosphates to its 3'-end. The TdT-labeling method can detect as little as 0.04 fmol of synthetic small RNA, and produce precise and accurate measurements that span a linear dynamic range from 0.04 to 5 fmol of synthetic small RNA |
721136 |
| 2.7.7.31 | biotechnology |
efficient production of recombinant enzyme in E. coli |
643233 |
| 2.7.7.31 | biotechnology |
use in production of synthetic homo- and heteropolymers, N-acetylation or N-alkylation of derivatives of dNTPs |
643199 |
| 2.7.7.31 | medicine |
little reliability of enzyme as marker of lymphoblastic lymphoma and leukemia |
643234 |
| 2.7.7.31 | medicine |
mechanism for inhibition of viral proliferation by L-nucleosides |
643225 |
| 2.7.7.31 | medicine |
use of enzyme in staining of apoptotic cells of leukemia cell lines |
643223 |
