EC Number |
Application |
Reference |
---|
2.7.1.17 | biotechnology |
putative use of lignocellulosic biomass as feedstock for the chemical industry |
690541 |
2.7.1.17 | synthesis |
an effective conversion of xylulose to xylulose 5-phosphate catalyzed by the xylulokinase in Saccharomyces cerevisiae is considered to be essential for the development of an efficient and accelerated ethanol fermentation process from xylulose |
645061 |
2.7.1.17 | synthesis |
commercial production of bioethanol from xylose using enzyme mutants with improved solubility expressed in presence of chaperonins GroEL-GroES |
661959 |
2.7.1.17 | synthesis |
construction of a Saccharomyces cerevisiae strain overexpressing GRE3-encoded NADPH-dependent aldose reductase and xylulokinase with a mutated strictly NADP+-dependent Pichia stipitis xylitol dehydrogenase. The recombinant strain efficiently ferments xylose and glucose mixture and the ethanol production is 21.4% higher than that of an isogenic constructed reference strain expressing Pichia stipitis xylose reductase. The yield of ethanol production increases from 0.395 g ethanol/g sugar to 0.435 g ethanol/g sugar after glucose depletion. Xylitol accumulation (0.6% of total sugar) is considerably lower than that of the reference strain (4.8% of total sugar) |
739564 |
2.7.1.17 | synthesis |
overexpression of enzyme, 3fold higher expression does not result in any increase in rate of growth or xylose metabolism |
661795 |
2.7.1.17 | synthesis |
silencing of gene expression with an antisense construct decreases D-xylulokinase activity after 48 h of incubation, leading to an increase in xylitol production from undetectable levels in wild-type to 8.6 mM |
737806 |
2.7.1.17 | synthesis |
xylulokinase and xylose isomerase are overexpressed in Klebsiella oxytoca HP1 to enhance hydrogen production by the fermentation of xylose. The recombinant strains exhibit higher enzyme activity compared with the wild-type strain. Hydrogen production from pure xylose, xylose/glucose mixtures and bamboo stalk hydrolysate is significantly enhanced. The hydrogen yield per mole substrate in strains expressing xylulokinase and xylose isomerase reaches 1.93 and 2.46 mol H2/mol xylose, respectively in pure xylose, while the value for the wild strain is 1.68 mol H2/mol xylose. Relative to the wild type, hydrogen yield from 1 g of preprocessed bamboo powder increases by 33.04 upon overexpression of xylulokinase |
-, 738412 |