EC Number |
Application |
Reference |
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2.3.1.94 | analysis |
kinetic assay for the megasynthase that stoichiometrically relates NADPH consumption to polyketide production |
736307 |
2.3.1.94 | biotechnology |
a derivative of Escherichia coli is genetically engineered to produce 6-deoxyerythronolide B, the macrocyclic core of the antibiotic erythromycin |
706754 |
2.3.1.94 | biotechnology |
a new Escherichia coli stain, YW9, is created, featuring a plasmid-free heterologous pathway for the production of the polyketide product 6-deoxyerythronolide B |
705513 |
2.3.1.94 | biotechnology |
polyketide synthases synthesize the polyketide cores of biologically active compounds, including natural products that have become important pharmaceuticals, like erythromycin |
706566 |
2.3.1.94 | synthesis |
the deoxyerythronolide B synthase genes are functionally expressed in Bacillus subtilis when the native eryAIIII operon is separated into three individual expression cassettes with optimized ribosomal binding sites. A synthesis of 6-deoxyerythronolide B can be detected by using the acetoininducible acoA promoter and a fed-batch simulating EnBase-cultivation strategy. Bacillus subtilis is capable of the secretion of 6-deoxyerythronolide B into the medium. The deletion of the prpBD operon of Bacillus subtilis, responsible for propionyl-CoA utilization, results in a significant increase of the 6-deoxyerythronolide B product yield when exogenous propionate is provided |
735515 |