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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.144synthesis by exploiting the substrate flexibility of both 4-coumaroyl:CoA ligase 4CL5 from Arabidopsis thaliana and HCBT, rapid biosynthesis of more than 160 cinnamoyl, dihydrocinnamoyl, and benzoyl anthranilates in yeast can be achieved upon feeding with both natural and non-natural cinnamates, dihydrocinnamates, benzoates, and anthranilates 737092
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.144synthesis coexpression of HCBT and 4-coumarate:CoA 4CL1 ligase from Nicotiana tabacum in the Escherichia coli anthranilate-accumulating strain W3110 trpD9923 allows the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] and Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid] upon feeding with p-coumarate and caffeate, respectively. Additional expression of a tyrosine ammonia-lyase from Rhodotorula glutinis (TAL) leads to the conversion of endogenous tyrosine into p-coumarate and results in the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] from glucose. A 135fold improvement in Avn D titer is achieved by boosting tyrosine production. Expression of either the p-coumarate 3-hydroxylase Sam5 from Saccharothrix espanensis or the hydroxylase complex HpaBC from Escherichia coli results in the endogenous production of caffeate and biosynthesis of Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid] 736747
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