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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122biotechnology an enzyme assay using the natural substrate trehalose dimycolate is developed. The colorimetric assay is based on the quantification of glucose from the degradation of trehalose, which is the product from catalytic activity of antigen 85A. This assay is suitable for robust high-throughput screening (HTS) for compound library screening against mycolyltransferase. The assay has a very low coefficient of variance (0.04) in 96-well plates and shows a Z' factor of 0.67-0.73, indicating the robustness of the assay 705100
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122drug development antigen 85 proteins are potential drug targets 720070
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.122medicine the enzyme is immunogenic in C57BL/6 mice and effective as tuberculosis vaccine, usage of vaccines combining mycolyl-transferase Ag85A and phosphate transport receptor PstS-3, overview 673960
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