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EC Number	Application	Commentary	Reference
1.5.99.13	analysis	an amperometric D-amino acid biosensor is prepared with a thermostable D-proline dehydrogenase and a carbon nanotube-ionic liquid gel	695578
1.5.99.13	analysis	development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane. The D-Pro DH/agar immobilized electrode is applied to determination of D-amino acids in a human urine sample	684424
1.5.99.13	energy production	a bio-anode using L-glutamate as the fuel is constructed. To oxidize L-glutamate at the anode, glutamate dehydrogenase, derived from Pyrobaculum islandicum, and proline dehydrogenase derived from Pyrococcus horikoshii, are immobilized for a two-enzyme conjugate enzymatic and redox reaction. To achieve an efficient enzyme reaction and electron transfer, the immobilization ratio of proline dehydrogenase to glutamate dehydrogenase is controlled by varying the molar ratios of dithiobis succinimidyl undecanoate and nitrilotriacetic acid dihydrochloride	747391

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Release 2023.1 (January 2023)