EC Number   |
Application |
Reference |
|---|
    1.5.3.13 | analysis |
assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Among the dansylpolyamines, N1,N11-didansylnorspermine is a useful substrate for the development of the N1-acetylpolyamine oxidase assay method. Contaminating polyamines in biological samples do not interfere with the method |
711352 |
| 1.5.3.13 | analysis |
assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Applied to cell extract from Chinese hamster ovary cell samples, the PAO activity can easily be detected with as little as 10 microg of protein, which corresponds to 1105 cells. The method is rapid, sensitive and useful. Contaminating polyamines in biological samples do not interfere with the method |
711352 |
| 1.5.3.13 | medicine |
high expression of APAO in A549 cells inhibits accumulation of antitumor polyamine analogue, N1-cyclopropylmethyl-N11-ethylnorspermine, decreases their sensitivity to the toxicity of N1-cyclopropylmethyl-N11-ethylnorspermine and prevents N1-cyclopropylmethyl-N11-ethylnorspermine-induced apoptosis |
711761 |
| 1.5.3.13 | pharmacology |
N,N'-butanedienyl butanediamine, i.e. MDL 72527 or CPC-200, a small molecule specific inhibitor of polyamine oxidase, effectively blocks androgen-induced reactive oxygen species production in human prostate cancer cells, as well as significantly delays prostate cancer progression and death in animals developing spontaneous prostate cancer |
703028 |
| 1.5.3.13 | synthesis |
application of simple guide molecules, being either covalently attached to polyamines or used as a supplement to the substrate mixtures, for controlling the enzyme's regioselectivity and stereospecificity |
764331 |
