1.5.1.54 | synthesis |
improvement of the biosynthesis of 5-MTHF by Lactococcus lactis by systematic pathway engineering, including the overexpression of the key rate-limiting enzyme MTHFR, strengthening 5-MTHF biosynthesis and folate supply by coexpression of the drfA, metF, and folE genes, increasing NADPH supply by overexpression of G6PDH, and increasing the proportion of 5-MTHF in various folates by overexpressing 5-formyltetrahydrofolate cyclo-ligase. The 5-MTHF titer finally reaches 300 microg/l with key precursor addition |
761689 |