   1.5.1.40 | synthesis |
Tfu-FNO variants can be used as NCB-Recycling systems for TsOYE-mediated conversions. TsOYE is known to efficiently hydrogenate ketoisophorone (2,6,6-trimethyl-2-cyclohexene-1,4-dione) to form the chiral product (R)-2,2,6- trimethylcyclohexane-1,4-dione with high enantiomeric excess values of 94-97%, when several NCBs as more efficient electron donors in comparison to NADPH are used. The Tfu-FNO variants G29Y, G29W, and P89H, which show high activity toward 1-aminoethylnicotinamide (AmNA+), 1-benzylnicotinamide (BNA+), and 1-benzyl-3-acetylpyridine (BAP+), are selected for use in conversion experiments as recycling catalysts for these NCBs. Potential use of these thermostable Tfu- FNO variants as NCB recycling systems at high temperatures |
763787 |
