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1.3.99.31
synthesis
swapping the native three-step CrtI in Rhodobacter sphaeroides,
EC 1.3.99.28
, for the four-step Pantoea agglomerans enzyme reroutes carotenoid biosynthesis and culminates in the production of 2,2'-diketo-spirilloxanthin under semi-aerobic conditions. Premature termination of this pathway by inactivating crtC or crtD produces strains with lycopene or rhodopin as major carotenoids. All of the spirilloxanthin series carotenoids are accepted by the assembly pathways for light-harvesting 2 complex and reaction centre-light-harvesting 1-PufX complexes
741967
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