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EC Number Application Commentary Reference
Show all pathways known for 1.2.7.7Display the word mapDisplay the reaction diagram Show all sequences 1.2.7.7synthesis engineering of Clostridium thermocellum to produce isobutanol. Both the native 2-oxoisovalerate-oxidoreductase KOR, EC 1.2.7.7, and the heterologous Lactococcus lactis 2-oxoisovalerate decarboxylase KIVD, EC 4.1.1.74, expressed are responsible for isobutanol production.The plasmid is integrated into the chromosome by single crossover. The resulting strain is stable without antibiotic selection pressure and produces 5.4g/l of isobutanol from cellulose in minimal medium at 50°C within 75 h, corresponding to 41% of theoretical yield -, 736738
Show all pathways known for 1.2.7.7Display the word mapDisplay the reaction diagram Show all sequences 1.2.7.7synthesis expression of a isobutanol synthesis pathway using ketoisovalerate ferredoxin oxidoreductase (Kor) from Clostridium thermocellum and bifunctional aldehyde/alcohol dehydrogenase (AdhE2) from Clostidium acetobutylicum. Only in recombinant Acetobacterium woodii strains, traces of isobutanol can be detected. Additional feeding of ketoisovalerate increases isobutanol production to 2.9 mM under heterotrophic conditions using isoform Kor3 and to 1.8 mM under autotrophic conditions using isoform Kor2. In Clostidium ljungdahlii, isobutanol can only be detected upon additional ketoisovalerate feeding under autotrophic conditions. Isoform Kor3 is the best suited gene cluster -, 763062
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