EC Number   |
Application |
Reference |
|---|
    1.2.4.1 | agriculture |
mutation of promoter binding protein SPL16/GW8 leads to upregulation of pyruvate kinase, pyruvate dehydrogenase E1, dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial pyruvate carrier, 4-hydroxyphenylpyruvate dioxygenase, and dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex. SPL16 mutations have the potential to boost the grain yield of rice |
763158 |
| 1.2.4.1 | biotechnology |
active expression of enzyme from non-halophilic Zymomonas mobilis in the haloarchaeon Haloferax volcanii with no difference in the secondary structure. Post-transcriptional mechanisms in the stationary phase appear to limit the amount of recombinant protein expressed |
663808 |
| 1.2.4.1 | medicine |
both subunits PDHA and PDHB are surface-exposed immunogenic proteins of Mycoplasma gallisepticum. The mouse anti-PDHA and anti-PDHB sera kill 48.0% and 75.1% of mycoplasmas, respectively. A combination of PDHA and PDHB antisera has a mean bactericidal rate of 65.2%. Both PDHA and PDHB adhere to DF-1 chicken embryo fibroblast cells and adherence is significantly inhibited by antisera against PDHA and PDHB. Both PDHA and PDHB are plasminogen-binding proteins |
-, 763621 |
| 1.2.4.1 | medicine |
high salt intake downregulates sirtuin SIRT3 level in brown adipose tissue, accompanied by decreased oxygen consumption rate, and causes a severe loss of brown adipose tissue characteristics. SIRT3 interacts with pyruvate dehydrogenase E1alpha (PDHA1) and deacetylates residue Lys83 both in vitro and in vivo under high salt intake. In parallel, high salt intake suppresses salt-induced kinase (Sik) 2 phosphorylation. Silencing Sik2 further diminishes SIRT3 activity and enhances acetylation of PDHA1 K83. Reconstruction of SIRT3 restores PDH activity and thermogenic markers expression in differentiated brown adipocytes from SIRT3 knockout mice |
762889 |
| 1.2.4.1 | medicine |
nitric oxide produced by murine macrophages is responsible for TCA cycle alterations and citrate accumulation associated with polarization. Inflammatory macrophages reroute pyruvate away from pyruvate dehydrogenase in an NO-dependent and hypoxia-inducible factor Hif1alpha-independent manner, thereby promoting glutamine-based anaplerosis |
763502 |
| 1.2.4.1 | medicine |
recombinant subunits PdhA and PdhB show binding activity with chicken plasminogen and human fibronectin. Rabbit anti-PdhA and anti-PdhB sera have distinct mycoplasmacidal efficacy in the presence of guinea pig complement, and the adherence of Mycoplasma synoviae to DF-1 cells pretreated with plasminogen is effectively inhibited by treatment with anti-rPdhA or anti-PdhB sera |
-, 763445 |
| 1.2.4.1 | synthesis |
coexpression of pyruvate dehydrogenase (PDH) E1 alpha and E1 beta subunitsin Escherichia coli leads to fully active E1 protein. The production of E1 alpha alone results in a catalytically inactive protein. The PDH E1 protein produced in Escherichia coli is capable of being phosphorylated by PDH-specific kinase |
758719 |
| 1.2.4.1 | synthesis |
engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase |
-, 713840 |
| 1.2.4.1 | synthesis |
production of pyruvate in an Escherichia coli strain with central metabolic pathways modified. Genes ldhA, pflB, pta-ackA, poxB, ppc, frdBC are knocked out sequentially and full pyruvate dehydrogenase is retained. In batch fermentation with M9 medium, pyruvate yield and production rate reach 0.63 g/g glucose and 1.89 g/(1 h), respectively. The production of acetate, succinate, and other carboxylates is effectively controlled, while the pathways of convertion of pyruvate to phosphoenol pyruvate and acetyl CoA are enhanced |
-, 763773 |
| 1.2.4.1 | synthesis |
when recombinant subunits PDHA and PDHB are mixed at a 1:1 molar ratio, they exhibit strong enzymatic activity. Alone, recombinant PDHA and PDHB exhibit no or weak enzymatic activity |
-, 763621 |
