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Results 1 - 10 of 11 > >>
EC Number Application Commentary Reference
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104analysis sensitive and rapid assay procedures for human mitochondrial the pyruvate dehydrogenase (PDH) complex, the 2-oxoglutarate dehydrogenase (OGDH) complex and their 5 component enzymes, for use with crude tissue extracts 759549
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104medicine enzyme/pyruvate dehydrogenase kinase dependent pathway is repressed in 73% of non-small cell lung carcinomas, which may be a key reason for hypoxia-inducible factor 1alpha stabilization and aerobic glycolysis. About half of enzyme-deficient carcinomas are not able to switch on the hypoxia-inducible factor 1alpha pathway, patients with these tumours have an excellent postoperative outcome. In contrast to cancer cells, fibroblasts in the tumour-supporting stroma exhibit an intense enzyme but reduced pyruvate dehydrogenase kinase 1 expression favoring maximum enzyme activity 662948
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104medicine high salt intake downregulates sirtuin SIRT3 level in brown adipose tissue, accompanied by decreased oxygen consumption rate, and causes a severe loss of brown adipose tissue characteristics. SIRT3 interacts with pyruvate dehydrogenase E1alpha (PDHA1) and deacetylates residue Lys83 both in vitro and in vivo under high salt intake. In parallel, high salt intake suppresses salt-induced kinase (Sik) 2 phosphorylation. Silencing Sik2 further diminishes SIRT3 activity and enhances acetylation of PDHA1 K83. Reconstruction of SIRT3 restores PDH activity and thermogenic markers expression in differentiated brown adipocytes from SIRT3 knockout mice 762889
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104medicine in a patient with primary lactic acidaemia, about 30% residual pyruvate dehydrogenase activity is observed, while the Km value is similiar to control. The enzyme deficiency is likely to be quantitative rather than qualitative. The patient developed severe metabolic acidosis at 8 months, accompanied by elevation of serum lactate and pyruvate. The lactate and pyruvate concentrations are also increased in cerevbrospinal fluid 759583
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104medicine nitric oxide produced by murine macrophages is responsible for TCA cycle alterations and citrate accumulation associated with polarization. Inflammatory macrophages reroute pyruvate away from pyruvate dehydrogenase in an NO-dependent and hypoxia-inducible factor Hif1alpha-independent manner, thereby promoting glutamine-based anaplerosis 763502
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104medicine the acute promyelocytic leukemia is greatly alleviated in the PDT-PAO-F16 treated group in an acute promyelocytic leukemia mice model 759077
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104synthesis coexpression of pyruvate dehydrogenase (PDH) E1 alpha and E1 beta subunits in Escherichia coli leads to fully active E1 protein. The production of E1 alpha alone results in a catalytically inactive protein. The PDH E1 protein produced in Escherichia coli is capable of being phosphorylated by PDH-specific kinase 758719
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104synthesis exchange of the native Corynebacterium glutamicum promoter of the AceE gene, with mutated DapA promoter variants leads to a series of strains with gradually reduced growth rates and pyruvate dehydrogenase complex activities. Upon overexpression of the L-valine biosynthetic genes IlvBNCE, all strains produce L-valine. Additional deletion of the Pqo and Ppc genes, encoding pyruvate:quinone oxidoreductase and phosphoenolpyruvate carboxylase enables production of up to 738mM (i.e., 86.5 g/liter). Inactivation of the transaminase B gene (IlvE) and overexpression of IlvBNCD instead of ilvBNCE transform the L-valine-producing strain into a 2-ketoisovalerate producer, excreting up to 303mM (35 g/liter) 2-ketoisovalerate. The replacement of the AceE promoter by the DapA-A16 promoter improves the production by 100% and 44%, respectively 758665
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104synthesis expression of pyruvate decarboxylase and alcohol dehydrogenase in Clostridium thermocellum DSM 1313. Though both enzymes are functional in Clostridium thermocellum, the presence of alcohol dehydrogenase severely limits the growth of the recombinant strains, irrespective of the presence or absence of the pyruvate decarboxylase gene. The recombinant strain expressing pyruvate decarboxylase shows two-fold increase in pyruvate carboxylase activity and ethanol production when compared with the wild type strain 735848
Show all pathways known for 1.2.1.104Display the reaction diagram Show all sequences 1.2.1.104synthesis metabolic engineering of Geobacillus thermoglucosidasius to divert the fermentative carbon flux from a mixed acid pathway to one in which ethanol becomes the major product, involving elimination of the lactate dehydrogenase and pyruvate formate lyase pathways by disruption of the ldh and pflB genes, respectively, and upregulation of expression of pyruvate dehydrogenase. Pyruvate dehydrogenase is active under anaerobic conditions, but expressed suboptimally for a role as the primary fermentation pathway. Strains with all three modifications form ethanol efficiently and rapidly at temperatures in excess of 60°C in yields in excess of 90% of theoretical. The strains also efficiently ferment cellobiose and a mixed hexose and pentose feed -, 736732
Results 1 - 10 of 11 > >>