EC Number |
Application |
Reference |
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1.14.18.4 | synthesis |
at the normal growth temperature of 30°C, Schizosaccharomyces pombe cells harboring FAH12 expression vector grow poorly when the FAH12 gene expression is induced. At 37°C, there is almost no growth inhibition. After preliminary growth at 37°C followed by a 5-day incubation at 20°C , the level of ricinoleic acid reaches 137.4 microg/ml of culture which corresponds to 52.6% of total fatty acids |
733184 |
1.14.18.4 | synthesis |
coexpression of fatty acid hydroxylase FAH and diacylglycerol acyl transferase DGAT1 in Pichia pastoris produces higher lipid contents and ricinoleic acid levels than expression of FAH alone. Coexpression in a mutant haploid strain defective in the DELTA12 desaturase activity results in a higher level of ricinoleic acid than that in the diploid strain. The ricinoleic acid produced is mainly distributed in the neutral lipid fractions, particularly the free fatty acid form, but with little in the polar lipids |
734438 |
1.14.18.4 | synthesis |
CpFAH, thet D12 oleate hydroxylase of nonplant origin, is a good candidate for the transgenic production of hydroxyl fatty acids in oilseed crops |
689616 |
1.14.18.4 | synthesis |
overexpression of multicopy suppressor Plg7, encoding phospholipase A2, in combinantion with oleate DELTA12-hydroxylase gene FAH12 enables Schizosaccharomyces pombe cells to secrete free ricinoleic acid into culture media. The FAH12 integrant in the absence of the overexpressed plg7 reaches 200 microg/ml of intracellular ricinoleic acid and only 69.3 microg/ml in culture media. The FAH12 integrant harboring the plg7 multicopy plasmid secretes ricinoleic acid in the media (184.5 microg/ml) without decreasing the amount in the cells |
733188 |